• Product NameHexokinase 1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificityHexokinase 1 Antibody detects endogenous levels of total Hexokinase 1
• Immunogen DescriptionA synthesized peptide derived from human Hexokinase 1
• Alternative Names    HK1;HK1-ta;HK1-tb;HK1-tc;HKI;HXK1; Hexokinase 1;
• Accession No. Uniprot:P19367
• UniprotP19367
• Calculated MW 102kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:200

• Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation.

Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation.

• Western blot analysis of Hexokinase 1 expression in (1) MCF-7 cell lysate; (2) 293T cell lysate.
• Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Hexokinase 1 Antibody.
• All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting