• Product NameCD8 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman
• SpecificityCD8 Antibody detects endogenous levels of total CD8
• Immunogen DescriptionA synthesized peptide derived from human CD8
• Alternative Names    CD8;T-cell surface glycoprotein CD8 alpha chain; Leu2; CD8A; MAL; T8 T cell antigen;
• Accession No. Uniprot:P01732
• UniprotP01732
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

• Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

• Western blot analysis of CD8 expression in Jurkat cell lysate.
• Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody.
• Immunofluorescent analysis of Jurkat cells, using CD8 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting