• Product NameKi67 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman
• SpecificityKi67 Antibody detects endogenous levels of total Ki67
• Immunogen DescriptionA synthesized peptide derived from human Ki67
• Alternative Names    MKI67; KIA; Antigen KI-67; Ki-67;
• Accession No. Uniprot:P46013
• UniprotP46013
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

• Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly(PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly(PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).

• Western blot analysis of Ki67 expression in Ramos cell lysate.
• Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody .
• Immunofluorescent analysis of Hela cells, using Ki67 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting