• Product NameCytokeratin 7 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman
• SpecificityCytokeratin 7 Antibody detects endogenous levels of total Cytokeratin 7
• Immunogen DescriptionA synthesized peptide derived from human Cytokeratin 7
• Alternative Names    CK-7; CK7; cytokeratin 7; Cytokeratin-7; K2C7; K7; keratin 7; Keratin-7; KRT7; Sarcolectin; SCL;
• Accession No. Uniprot:P08729
• UniprotP08729
• Calculated MW 51kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20

• K7 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

K7 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

• Western blot analysis of Cytokeratin 7 expression in T47 D cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cytokeratin 7 Antibody .
• Immunofluorescent analysis of Hela cells, using Cytokeratin 7 Antibody.
• All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting