• Product NameHistone H3.3 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityHistone H3.3 Antibody detects endogenous levels of total Histone H3.3
• Immunogen DescriptionA synthesized peptide derived from human Histone H3.3
• Alternative Names    H3.3; H3.3A; H33_HUMAN ; H3F3; Histone H3.3 ; H3 histone family 3A; H3 histone family 3B
• Accession No. Uniprot:P84243
• UniprotP84243
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:500~1:1000 ICC/IF 1:500~1:1000

• Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

• Western blot analysis of Histone H3.3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
• Immunohistochemical analysis of paraffin-embedded mouse stomach, using Histone H3.3 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting