• Product Name5T4 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman
• Specificity5T4 Antibody detects endogenous levels of 5T4
• Immunogen DescriptionA synthesized peptide derived from human 5T4
• Alternative Names    TPBG; 5T4; 5T4-AG; 5T4AG; 5T4 oncofetal antigen; M6P1; Trophoblast glycoprotein;
• Accession No. Uniprot:Q13641
• UniprotQ13641
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:200

• 5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1�C60.

5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1�C60.

• Western blot analysis of 5T4 expression in Caco-2 cell lysates.
• Immunohistochemical analysis of paraffin-embedded human uters cancer, using 5T4 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting