• Product NameChk2 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman
• SpecificityChk2 Antibody detects endogenous levels of Chk2
• Immunogen DescriptionA synthesized peptide derived from human Chk2
• Alternative Names    CHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1; RAD53; kinase Chk2;
• Accession No. Uniprot:O96017
• UniprotO96017
• Calculated MW 62kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:10000~1:20000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

• These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

• Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate.
• Immunohistochemical analysis of paraffin-embedded human testis, using Chk2 Antibody.
• Immunofluorescent analysis of Hela cells, using Chk2 Antibody.
• All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting