• Product NameIKK beta Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IP
• Species ReactivityHuman Mouse
• SpecificityIKK beta Antibody detects endogenous levels of IKK beta
• Immunogen DescriptionA synthesized peptide derived from human IKK beta
• Alternative Names    EC 2.7.11.10; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-B; IKK-beta; IKK2; IKKB; IkBKB; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; kinase IKK-beta;
• Accession No. Uniprot:O14920
• UniprotO14920
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IP 1:50

• The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

• Western blot analysis of IKK beta expression in Daudi cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting