• Product NameRetinoblastoma Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse
• SpecificityRetinoblastoma Antibody detects endogenous levels of Retinoblastoma
• Immunogen DescriptionA synthesized peptide derived from human Retinoblastoma
• Alternative Names    OSRC; RB; p105-Rb; RB1; pRb; pp110;
• Accession No. Uniprot:P06400
• UniprotP06400
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:100~1:500 ICC/IF 1:100~1:500 IP 1:50

• The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression.

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression.

• Western blot analysis of Retinoblastoma expression in (1) Jurkat cell lysate; (2) MCF-7 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human lung cancer, using Retinoblastoma Antibody.
• Immunofluorescent analysis of SH-SY5Y cells, using Retinoblastoma Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting