• Product Namecleaved Caspase-9 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IP
• Species ReactivityHuman Mouse
• Specificitycleaved Caspase-9 Antibody detects endogenous levels of cleaved Caspase-9
• Immunogen DescriptionA synthesized peptide derived from human cleaved Caspase-9
• Alternative Names    MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; CASP9; Caspase-9; Caspase 9 Dominant Negative;
• Accession No. Uniprot:P55211
• UniprotP55211
• Calculated MW 46,39,37,35kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IP 1:50

• Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

• Western blot analysis of cleaved Caspase-9 Antibody expression in HeLa cell lysate treated with staurosporine.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting