• Product NameCytokeratin 14 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityCytokeratin 14 Antibody detects endogenous levels of Cytokeratin 14
• Immunogen DescriptionA synthesized peptide derived from human Cytokeratin 14
• Alternative Names    CK-14; CK14; cytokeratin 14; Cytokeratin-14; EBS3; EBS4; K14; K1C14; keratin 14; Keratin, type I cytoskeletal 14; Keratin-14; KRT14; NFJ;
• Accession No. Uniprot:P02533
• UniprotP02533
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

• K14 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

K14 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

• Western blot analysis of Cytokeratin 14 expression in A431 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human stomach, using Cytokeratin 14 Antibody.
• Immunofluorescent analysis of HACAT cells, using Cytokeratin 14 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting