• Product NameSmac/Diablo Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificitySmac/Diablo Antibody detects endogenous levels of Smac/Diablo
• Immunogen DescriptionA synthesized peptide derived from human Smac/Diablo
• Alternative Names    SMAC; DFNA64; DIABLO; SMAC3;
• Accession No. Uniprot:Q9NR28
• UniprotQ9NR28
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50 FC 1:50

• Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases.

Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases.

• Western blot analysis of Smac/Diablo expression in Jurkat cell lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using Smac/Diablo Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting