• Product NameMyosin Light Chain 2 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC IP
• Species ReactivityHuman Mouse Rat
• SpecificityMyosin Light Chain 2 Antibody detects endogenous levels of Myosin Light Chain 2
• Immunogen DescriptionA synthesized peptide derived from human Myosin Light Chain 2
• Alternative Names    MYL2; MLC2; Myosin light chain 2; MLC-2; RLC of myosin; CMH10; MLC-2v;
• Accession No. Uniprot:P10916
• UniprotP10916
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:5000 IHC 1:50~1:200 IP 1:50

• Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner.

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner.

• Western blot analysis of Myosin Light Chain 2 expression in Mouse heart lysate.
• Immunohistochemical analysis of paraffin-embedded human heart, using Myosin Light Chain 2 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting