• Product NameBTK Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IP
• Species ReactivityHuman
• SpecificityBTK Antibody detects endogenous levels of BTK
• Immunogen DescriptionA synthesized peptide derived from human BTK
• Alternative Names    AGMX1; Tyrosine-protein kinase BTK; BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; ATK; BPK;
• Accession No. Uniprot:Q06187
• UniprotQ06187
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IP1:20

• Btk plays an important role in B lymphocyte development, differentiation and signaling. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223.

Btk plays an important role in B lymphocyte development, differentiation and signaling. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223.

• Western blot analysis of BTK expression in (1) Daudi cell lysate; (2) Ramos cell lysate; (3) K562 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BTK Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting