• Product NameOct4 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP ChIP
• Species ReactivityHuman Mouse
• SpecificityOct4 Antibody detects endogenous levels of Oct4
• Immunogen DescriptionA synthesized peptide derived from human Oct4
• Alternative Names    Octamer binding protein 3; Octamer binding protein 4; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; POU5F1;
• Accession No. Uniprot:Q01860
• UniprotQ01860
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 ChIP

• Oct-4 is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Oct-4 is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

• Western blot analysis of Oct4 expression in NCCIT cell lysate.
• Immunohistochemical analysis of paraffin-embedded human seminoma, using Oct4 Antibody.
• Immunofluorescent analysis of NCCIT cells, using Oct4 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting