• Product NameCaspase-3 p12 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityCaspase-3 p12 Antibody detects endogenous levels of Caspase-3 p12
• Immunogen DescriptionA synthesized peptide derived from human Caspase-3 p12
• Alternative Names    Apopain; CASP-3; CASP3; Caspase-3 subunit p12; CPP-32; Cysteine protease CPP32; Protein Yama; SCA-1; SREBP cleavage activity 1;
• Accession No. Uniprot:P42574
• UniprotP42574
• Calculated MW 12,29,32kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:100~1:500 ICC/IF 1:50~1:200

• Caspase-3 is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Involved in the activation cascade of caspases responsible for apoptosis execution.

Caspase-3 is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Involved in the activation cascade of caspases responsible for apoptosis execution.

• Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using Caspase-3 p12 Antibody.
• Immunofluorescent analysis of HeLa cells, using Caspase-3 p12 Antibody.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting