• Product NameCyclin E2 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse
• SpecificityCyclin E2 Antibody detects endogenous levels of Cyclin E2
• Immunogen DescriptionA synthesized peptide derived from human Cyclin E2
• Alternative Names    CCN E2; CCNE 2; CCNE2 protein; CYC E2; CYCE 2; CyclinE2; G1/S specific cyclin E2;
• Accession No. Uniprot:O96020
• UniprotO96020
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:100~1:500 FC 1:20

• Cyclin E1 and cyclin E2 can associate with and activate CDK2. Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. Essential for the control of the cell cycle at the late G1 and early S phase.

Cyclin E1 and cyclin E2 can associate with and activate CDK2. Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. Essential for the control of the cell cycle at the late G1 and early S phase.

• Western blot analysis of Cyclin E2 expression in HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin E2 Antibody.
• Immunofluorescent analysis of Hela cells, using Cyclin E2 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting