• Product NamePDPK1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityPDPK1 Antibody detects endogenous levels of PDPK1
• Immunogen DescriptionA synthesized peptide derived from human PDPK1
• Alternative Names    3-phosphoinositide dependent protein kinase-1; EC 2.7.11.1; hPDK1; kinase PDK1; mPDK1; PDPK1; PkB kinase; Protein kinase B kinase;
• Accession No. Uniprot:O15530
• UniprotO15530
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:100~1:500 ICC/IF 1:50~1:200

• Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK. Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK. Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

• Western blot analysis of PDPK1 expression in HEK293 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using PDPK1 Antibody.
• Immunofluorescent analysis of PC-12 cells, using PDPK1 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting