• Product NameMEK7 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityMEK7 Antibody detects endogenous levels of MEK7
• Immunogen DescriptionA synthesized peptide derived from human MEK7
• Alternative Names    MKK7; Jnkk2; MAPKK7; PRKMK7; JNK-activating kinase 2; MAPKK 7; Mitogen Activated Protein Kinase kinase 7; SAPKK4; stress-activated protein kinase kinase 4;
• Accession No. Uniprot:O14733
• UniprotO14733
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:40 FC 1:50

• MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α. Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α. Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

• Western blot analysis of MEK5 expression in Hela cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting