• Product NameATF4 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityATF4 Antibody detects endogenous levels of ATF4
• Immunogen DescriptionA synthesized peptide derived from human ATF4
• Alternative Names    Cyclic AMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67;
• Accession No. Uniprot:P18848
• UniprotP18848
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:200 ICC 1:100~1:500 IP 1:50 FC 1:50

• ATF4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway. ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4. Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress.

ATF4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway. ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4. Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress.

• Western blot analysis of ATF4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
• Immunohistochemical analysis of paraffin-embedded mouse kidney, using ATF4 Antibody.
• Immunofluorescent analysis of 293 cells, using ATF4 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting