• Product NameHistone H4 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF ChIP
• Species ReactivityHuman Mouse Rat
• SpecificityHistone H4 Antibody detects endogenous levels of Histone H4
• Immunogen DescriptionA synthesized peptide derived from human Histone H4
• Alternative Names    Histone H4; HIST1H4A; H4/A, H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G, H4FG; HIST1H4D; H4/B, H4FB; HIST1H4E; H4/J, H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E, H4FE; HIST1H4K; H4/D, H4FD; HIST1H4L; H4/K; H4FK;
• Accession No. Uniprot:P62805
• UniprotP62805
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:100~1:500 ICC/IF 1:50~1:200 IP 1:50 FC 1:50 ChIP

• Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family.

• Western blot analysis of Histone H4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human uterus, using Histone H4 Antibody.
• Immunofluorescent analysis of Hela cells, using Histone H4 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting