• Product NameSTAT5b Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificitySTAT5b Antibody detects endogenous levels of STAT5b
• Immunogen DescriptionA synthesized peptide derived from human STAT5b
• Alternative Names    Signal transducer and activator of transcription 5B; STA5B; STAT5; Stat5b; Transcription factor STAT5B;
• Accession No. Uniprot:P51692
• UniprotP51692
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:1000~1:2000 ICC/IF 1:1000~1:2000 IP 1:40

• Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types.

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types.

• Western blot analysis of STAT5b expression in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) C6 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast, using STAT5b Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting