• Product NameHDAC4 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.20E12
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF
• Species ReactivityHuman
• SpecificityHDAC4 Antibody detects endogenous levels of HDAC4
• Immunogen DescriptionA synthesized peptide derived from human HDAC4
• Target NameHDAC4
• Alternative Names    Histone deacetylase 4; HD4; HDAC4; AHO3; BDMR;
• Accession No. Uniprot:P56524
• UniprotP56524
• Calculated MW 140kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 ICC/IF 1:50~1:200

• Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

• All Lanes: HDAC4 Antibody at 1/ 500 dilution.Lane1:Hela lysate.Proteins at 20ug per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 140kDaObserved band size: 140kDa
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting