• Product NameCDC34 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityCDC34 Antibody detects endogenous levels of total CDC34
• Immunogen DescriptionA synthesized peptide derived from human CDC34
• Alternative Names    CDC34; Cell division cycle 34; UBC3; UBCH3; Ubiquitin-protein ligase R1; UBE2R1; E2-CDC34; Ubiquitin carrier protein;
• Accession No. Uniprot:P49427
• UniprotP49427
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

• Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation.

Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation.

• Western blot analysis of CDC34 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
• Immunofluorescent analysis of Hela cells, using CDC34 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting