• Product NamePD-L1 (CD274) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman Mouse Rat
• SpecificityPD-L1 (CD274) Antibody detects endogenous levels of total PD-L1 (CD274)
• Immunogen DescriptionA synthesized peptide derived from human PD-L1 (CD274)
• Alternative Names    B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; CD274;
• Accession No. Uniprot:Q9NZQ7
• UniprotQ9NZQ7
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:100~1:500 IHC 1:50~1:100

• Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens.

Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens.

• Western blot analysis of PD-L1 (CD274) expression in Ramos cell lysate.
• Immunohistochemical analysis of paraffin-embedded human thymoma, using PD-L1 (CD274) Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting