• Product NameKMT6 / EZH2 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificityKMT6 / EZH2 Antibody detects endogenous levels of total KMT6 / EZH2
• Immunogen DescriptionA synthesized peptide derived from human KMT6 / EZH2
• Alternative Names    EZH 2; EZH1; EZH2; EZH2b; KMT 6; KMT6; KMT6A;
• Accession No. Uniprot:Q15910
• UniprotQ15910
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

• Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

• Western blot analysis of KMT6 / EZH2 expression in HEK293 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human tonsil, using KMT6 / EZH2 Antibody.
• Immunofluorescent analysis of Hela cells, using KMT6 / EZH2 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting