• Product NameLC3B Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;ICC/IF;IHF;IP
• Species ReactivityHuman Mouse Rat
• SpecificityLC3B Antibody detects endogenous levels of total LC3B
• Immunogen DescriptionA synthesized peptide derived from human LC3B
• Alternative Names    ATG8F; LC3B; MAP1LC3B; MLP3B; MAP1LC3B;
• Accession No. Uniprot:Q9GZQ8
• UniprotQ9GZQ8
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000-1:2000
  IHC 1:100-1:200 ICC/IF 1:50-1:200 IP 1:20-1:50   

• Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.

• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
• Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.
• Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:200 dilution.
• Immunofluorescent analysis of Hela cells treated with choroquine, using LC3B Antibody.
• Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry
• Immunofluorescent analysis using the Antibody at 1:150 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting