• Product NameBid Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman
• SpecificityBid Antibody detects endogenous levels of total Bid
• Immunogen DescriptionA synthesized peptide derived from human Bid
• Alternative Names    BID isoform ES(1b); BID isoform L(2); BID isoform Si6; FP497; p11 BID; p13 BID; p15 BID; p22 BID;
• Accession No. Uniprot:P55957
• UniprotP55957
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:10 FC 1:50

• The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

• Western blot analysis of Bid expression in Jurkat cell lysate.
• Immunohistochemical analysis of paraffin-embedded human lung, using Bid Antibody.
• Immunofluorescent analysis of Jurkat cells, using Bid Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting