• Product NameS100 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC IP FC
• Species ReactivityHuman Mouse
• SpecificityS100 Antibody detects endogenous levels of total S100
• Immunogen DescriptionA synthesized peptide derived from human S100
• Alternative Names    Bpb; NEF; Protein S100-A1; S100 beta; S100 calcium binding protein A1; S100A; S100B; 100beta;
• Accession No. Uniprot:P23297
• UniprotP23297
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:1000 IHC 1:100~1:500 IP 1:50 FC 1:50

• Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

• Western blot analysis of S100 expression in (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using S100 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting