• Product NameDrosha Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman
• SpecificityDrosha Antibody detects endogenous levels of total Drosha
• Immunogen DescriptionA synthesized peptide derived from human Drosha
• Alternative Names    Ribonuclease 3 (EC:3.1.26.3); Protein Drosha; Ribonuclease III; RNase III; p241; DROSHA; RN3; RNASE3L; RNASEN;
• Accession No. Uniprot:Q9NRR4
• UniprotQ9NRR4
• Calculated MW 159kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

• Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing. This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs. Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8. Drosha, along with DGCR8, is necessary for miRNA biogenesis.

Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing. This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs. Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8. Drosha, along with DGCR8, is necessary for miRNA biogenesis.

• Western blot analysis of Drosha expression in 293 cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting