• Product NameOXGR1 / GPR99 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IF
• Species ReactivityHuman;Mouse;Rat
• SpecificityOXGR1 / GPR99 Antibody detects endogenous levels of total OXGR1 / GPR99
• Immunogen DescriptionA synthesized peptide derived from human OXGR1 / GPR99
• Alternative Names    GPR80; GPR99; Oxgr1; P2RY15; P2Y15;
• Accession No. Uniprot:Q96P68
• UniprotQ96P68
• Calculated MW 38 kDa
• SDS-PAGE MW 38 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:50

• Receptor for alpha-ketoglutarate. Seems to act exclusively through a G(q)-mediated pathway.

Receptor for alpha-ketoglutarate. Seems to act exclusively through a G(q)-mediated pathway.

• All lanes : OXGR1 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Hela whole cell lysates
  Lane 2 : A549 whole cell lysates
  Lane 3 : HUVEC whole cell lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 38 kDa
  Observed band size: 38 kDa
  
  Exposure time: 10 seconds
• All lanes : OXGR1 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Mouse Kidney lysates
  Lane 2 : Rat Spleen lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 38 kDa
  Observed band size: 38 kDa
  
  Exposure time: 45 seconds
• All lanes : OXGR1 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Wild-type Hela cell lysate
  Lane 2 : OXGR1 knockdown Hela cell lysate
  
  Lysates/proteins at 20 µg per lane.
• Immunocytochemistry/ Immunofluorescence OXGR1 antibody (60143)
  
  60143 staining OXGR1 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
  Samples were incubated with 60143 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
  The negative control is shown in bottom right hand panel - for the negative control, purified 60143 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
  Nuclei were counterstained with DAPI.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting