• Product NamePhospho-PKC alpha (T497) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthesized peptide derived from human Phospho-PKC alpha (T497)
• UniprotP17252
• Calculated MW 77kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

•  WB 1:500~1:2000 IHC 1:50~1:200

 PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.

• Western blot analysis of Phospho-PKC alpha (T497) expression in HeLa treated with Calyculin A cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting