• Product NamePhospho-Src (Y529) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB,ICC/IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthesized peptide derived from human Phospho-Src (Y529)
• UniprotP12931
• Calculated MW 60kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

•  WB 1:500~1:2000 ICC/IF 1:50~1:200

 Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function.

• Western blot analysis of Phospho-Src (Y529) expression in A431 cell treated with EGF lysate.
• All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting