• Product NamePaxillin(phospho-Tyr88) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 88.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.
• Target NamePaxillin
• ModificationPhospho
• Alternative Names    PAXI; PXN;
• Accession No. Swiss-Prot: P49023
  NCBI Protein: NP_001074324.1
• UniprotP49023
• Gene ID5829;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 68kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.

• Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody #11538.
• Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody #11538.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting