• Product NameNPTII/Neomycin Phosphotransferase 2 antibody
• Brief DescriptionRabbit Polyclonal
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity purified by Protein A.
• ApplicationsWB, IHC-P, IHC-F, IF, ICC/IF, ELISA
• Species ReactivityKlebsiella pneumoniae
• Immunogen TypePeptide
• Immunogen Descriptionpeptide derived from Klebsiella pneumoniae Neomycin Phosphotransferase 2:?101-200/264
• Target NameNPTII/Neomycin Phosphotransferase 2
• UniprotP00552
• Calculated MW 32 kDa
• Concentration 1mg/ml
• Formulation Liquid in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
• Storage Store at +4˚C for short term. Store at -20˚C for long term. Avoid freeze/thaw cycle.

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting