• Product NameGLS1 antibody
• Brief DescriptionRabbit Polyclonal
• Host SpeciesRabbit
• ClonalityPolyclonal
• Purificationaffinity purified by Protein A.
• ApplicationsWB, IHC, IF, ELISA
• Species ReactivityHuman, Mouse, Rat
• SpecificityThis antibody detects endogenous levels of ZN558.
• Immunogen DescriptionKLH conjugated synthetic peptide derived from human GLS1: 141-240/669
• Target NameGLS1
• UniprotO94925
• Gene ID2744
• Calculated MW 72 kDa
• Concentration 1mg/ml
• Formulation 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB 1:500-2000, IHC-P 1:100-500, IHC-F 1:100-500, IF 1:100-500, ELISA 1:5000-10000

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].

• Western blot analysis of lysates using GLS1 antibody at 1/1000 dilution Lane 1: Human U251 cell lysates Lane 2: Human HeLa cell lysates
• Immunohistochemical analysis of paraffin-embedded Rat brain with GLS1 Antibody at 1:400.
• Immunohistochemical analysis of paraffin-embedded Mouse brain with GLS1 Antibody at 1:400.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting