• Product NameFAK(phospho-Tyr576/Tyr577) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
• Target NameFAK
• ModificationPhospho
• Alternative Names    FADK 1; FAK1; PTK2
• Accession No. Swiss-Prot: Q05397
  NCBI Protein: NP_005598.3
• UniprotQ05397
• Gene ID5747;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 125kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613
Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.

• Western blot analysis of extracts from HT29 cells untreated or treated with PMA using FAK(phospho-Tyr576/Tyr577) Antibody #11545.
• Immunofluorescence staining of methanol-fixed Hela cells using FAK(phospho-Tyr576/Tyr577) Antibody #11545.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting