• Product NameEZH2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu Ms
• SpecificityMultiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 17 amino acid peptide near the amino terminus of human EZH2.
• Target NameEZH2
• Alternative Names    Enhancer of zeste homolog 2, histone-lysine-N-methylase EZH2, KMT6A, ENX1
• Accession No. Swiss-Prot:Q15910
  Gene ID:2146
• UniprotQ15910
• Gene ID2146;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.

• Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting