• Product NameNUMB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu Ms Rt
• SpecificityAt least four isoforms of NUMB are known to exist; this antibody will detect all of them.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 17 amino acid peptide near the carboxy terminus of human NUMB.
• Target NameNUMB
• Alternative Names    m-NUMB, S171
• Accession No. Swiss-Prot:P49757
  Gene ID:8650
• UniprotP49757
• Gene ID8650;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

• Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting