• Product NameCdc25B(Phospho-Ser149) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityMs
• SpecificityThe antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
• Target NameCdc25B
• ModificationPhospho
• Alternative Names    CDC25B
• Accession No. Swiss-Prot:P30306
  Gene ID:12531
• UniprotP30306
• Gene ID12531;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 62kd
  Western blotting: 1:500~1:1000

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Li G, et al. (2010) Retrovirology.7:59.
Wang Z,et al. (2010) BMC Cancer.10:233.
Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.

• Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody #11553.
• Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody #11553.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting