• Product NameLEMD1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu Ms
• SpecificityAt least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 19 amino acid peptide near the center of human LEMD1.
• Target NameLEMD1
• Alternative Names    LEM domain containing 1, Cancer, testis antigen 50, CT50, LEMP-1
• Accession No. Q68G75
• UniprotQ68G75
• Gene ID93273;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.

• Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting