• Product NameRaf1(Ab-259) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Raf1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.257~261 (S-T-S-T-P) derived from Human RAF.
• Target NameRaf1
• Alternative Names    C-RAF; C-Raf; CRAF
• Accession No. Swiss-Prot: P04049
  NCBI Protein: NP _002871.1
• UniprotP04049
• Gene ID5894;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 73kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.
Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.

• Western blot analysis of extracts from Hela, K562 and HT29 cells using Raf1(Ab-259) Antibody #21006.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Ab-259) Antibody #21006.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting