• Product NameGSK3a(Ab-21) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total GSK3a protein. and does not detect GSK-3b protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.19~23 (T-S-S-F-A) derived from Human GSK3a.
• Target NameGSK3a
• Alternative Names    Factor A, GSK-3 alpha, kinase GSK3-alpha,
• Accession No. Swiss-Prot: P49840
  NCBI Protein: NP_063937.2
• UniprotP49840
• Gene ID2931;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 51kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN

Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Cross D A, et al. (1995) Nature. 378: 785-789.

• Western blot analysis of extracts from 293 cells using GSK3a(Ab-21) Antibody #21007.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a(Ab-21) Antibody #21007(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF7 cells using GSK3a(Ab-21) Antibody #21007.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting