• Product NameMEK2(Ab-394) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous level of total MEK2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.392~396 (P-G-T-P-T) derived from Human MEK-2.
• Target NameMEK2
• Alternative Names    ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2
• Accession No. Swiss-Prot: P36507
  NCBI Protein: NP_109587.1
• UniprotP36507
• Gene ID5605;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 44kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases

Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.

• Western blot analysis of extracts from Hela, HepG2, PC12 and HUVEC cells using MEK-2(Ab-394) Antibody #21008.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Ab-394) Antibody #21008(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting