NFkB-p65(Ab-536) Antibody - SAB | Signalway Antibody

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NFkB-p65(Ab-536) Antibody #21014

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Catalog No.	Package Size	Price	Quantity
21014-1	50ul	$219.00
21014-2	100ul	$319.00

Availability:Yes

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Description

Product NameNFkB-p65(Ab-536) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total NFkB-p65 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.534~538 (F-S-S-I-A) derived from Human NFkB-p65.
Target NameNFkB-p65
Alternative Names NFKB3; RELA; TF65; Transcription factor p65; p65

Accession No. Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1
UniprotQ04206
Gene ID5970;
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501.
Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.

Published Papers

5-HT2A Receptor and 5-HT Degradation Play a Crucial Role in Atherosclerosis by Modulating Macrophage Foam Cell Formation, Vascular Endothelial Cell Inflammation, and Hepatic Steatosis In J Atheroscler Thromb on 2022 Mar 1 by Yingying Ma, Xiurui Liang,et al..PMID: 33536397, , (2022),
PMID: 33536397 The A-to-I editing of KPC1 promotes intrahepatic cholangiocarcinoma by attenuating proteasomal processing of NF-κB1 p105 to p50. In J Exp Clin Cancer Res on 2022 Dec 8 by Chengming Gao, Guangming Zhou, et al..PMID:36476255, , (2022),
PMID: 36476255 5-HT2A Receptor and 5-HT Degradation Play a Crucial Role in Atherosclerosis by Modulating Macrophage Foam Cell Formation, Vascular Endothelial Cell Inflammation, and Hepatic Steatosis. In J Atheroscler Thromb on 2021 Feb 2 by Yingying Ma, Xiurui Liang,et al..PMID: 33536397, , (2021),
PMID: 33536397 Alpha-lipoic acid alleviates NAFLD and triglyceride accumulation in liver via modulating hepatic NLRP3 inflammasome activation pathway in type 2 diabetic rats. In Food Sci Nutr on 2021 Mar 13 by Chih-Yuan Ko, Yangming Martin Lo, et al..PMID: 34026086, , (2021),
PMID: 34026086 View All Published Papers CYP4A11 is involved in the development of nonalcoholic fatty liver disease via ROS?induced lipid peroxidation and inflammation.In Int J Mol Med on 2020 Apr by Gao H, Cao Y, et al..PMID:32124935, , (2020),
PMID: 32124935 Phytoestrogens protect joints in collagen induced arthritis by increasing IgG glycosylation and reducing osteoclast activation.In Int Immunopharmacol on 2020 Mar 12 by Du N, Song L, et al..PMID:32172207, , (2020),
PMID: 32172207 Loranthus Micranthus Extracts Suppress the Expression and Enzymatic Activity of Indoleamine 2, 3-Dioxygenase in Human Breast Cancer Cells via the Inhibition of JAK/STAT and NF-kB Pathways, , (2020),
PMID: Artemether Activation of AMPK/GSK3β(ser9)/Nrf2 SignalIng Confers Neuroprotection towards β-Amyloid-Induced Neurotoxicity In 3xTg Alzheimer's Mouse Model. In Oxid Med Cell Longev on 2019 Nov 21 by Li S, Zhao X, et al..PMID:31871541, , (2019),
PMID: 31871541 Arctigenin suppresses renal interstitial fibrosis in a rat model of obstructive nephropathy.In Phytomedicine on 2017 Jul 1 by Ao Li , Xiaoxun Zhang,et al..PMID: 28545667, , (2017),
PMID: 28545667 Endoplasmic reticulum stress participates in inflammation-accelerated, lipid-mediated injury of human glomerular mesangial cells.In Nephrology (Carlton) on 2017 Mar by Haiping Yang, Jingjing Cui,et al..PMID: 26890338, , (2017),
PMID: 26890338 Pteisolic acid G, a novel ent?kaurane diterpenoid, inhibits viability and induces apoptosis in human colorectal carcinoma cells.In Oncol Lett on 2017 Nov by Shuangli Qiu ,Xin Wu,et al..PMID: 29113182, , (2017),
PMID: 29113182 Spautin-1 ameliorates acute pancreatitis via inhibiting impaired autophagy and alleviating calcium overload.In Mol Med on 2016 Oct by Juan Xiao , Xueping Feng et al..PMID:27579473, , (2016),
PMID: 27579473 Protodioscin ameliorates fructose-induced renal injury via inhibition of the mitogen activated protein kinase pathway.In Phytomedicine on 2016 Nov 15 by Jinyang Shen, Xiaolin Yang et al..PMID:27765371, , (2016),
PMID: 27765371 Neonatal chlorpyrifos exposure induces loss of dopaminergic neurons in young adult rats.In Toxicology on 2015 Oct 2 by Jie Zhang , Hongmei Dai et al..PMID: 26215101, , (2015),
PMID: 26215101 Role of toll-like receptor 4 in mediating multiorgan dysfunction in mice with acetaminophen induced acute liver failure. In Liver Transpl on 2013 Jul by Maria Jover-Cobos, Ken-Ichi Tanamoto, et al..PMID:23554142 , , (2013),
PMID: 23554142 Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression.In J Hepatol on 2012 May by Shah N, Dhar D, et al..PMID:22266601, , (2012),
PMID: 22266601 Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987, , (2012),
PMID: 23191987 Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression.In J Hepatol on 2012 May by Shah N, Dhar D, et al..PMID:22266601, , (2012),
PMID: 22266601 Exendin-4 directly improves endothelial dysfunction in isolated aortas from obese rats through the cAMP or AMPK-eNOS pathways.In Diabetes Res Clin Pract on 2012 Sep by Han L, Yu Y,et al..PMID:22560150, , (2012),
PMID: 22560150 Dietary Oxidized n-3 PUFA Induce Oxidative Stress and Inflammation: Role of Intestinal Absorption of 4-HHE and Reactivity in Intestinal Cells.In J Lipid Res on 2012 Oct by Manar Awada, Christophe O Soulage,et al..PMID:22865918, , (2012),
PMID: 22865918 Peroxisome proliferator activated receptor alpha activation decreases inflammatory and destructive responses in osteoarthritic cartilage.In Osteoarthritis Cartilage on 2011 Jul by Clockaerts S, Bastiaansen-Jenniskens YM,et al..PMID:21458577, , (2011),
PMID: 21458577 Please let us know if you have published research using #21014 so that we can cite your reference.

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NFkB-p65(Ab-536) Antibody(21014)

NOTE

Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse

RtRat

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine

D Dog

PPig

HmHamster

ChHm Chinese Hamster

ChkChicken

ShpSheep