• Product NameRel(Ab-503) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsIF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Rel protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.501~505 (T-S-S-D-S) derived from Human Rel.
• Target NameRel
• Alternative Names    C-Rel
• Accession No. Swiss-Prot: Q04864
  NCBI Protein: NP_002899.1
• UniprotQ04864
• Gene ID5966;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 78kd
  Immunofluorescence: 1:100~1:200

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 (PubMed18037997)).

Copley RR et al. (2007) Genome Res. 17(9):1327-35.
Liu YW,et al.(2007) Cell Mol Life Sci. 64(24):3282-94.
Banerjee D, (2005) Immunity. 23(4):445-58.

• Immunofluorescence staining of methanol-fixed Hela cells using Rel(Ab-503) Antibody #21020.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting