• Product Namec-Jun(Ab-91) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total c-Jun protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.89~93 (T-T-T-P-T) derived from Human c-Jun.
• Target Namec-Jun
• Alternative Names    AH119; AP1; Jun A; c-Jun; p39
• Accession No. Swiss-Prot: P05412
  NCBI Protein: NP_002219.1
• UniprotP05412
• Gene ID3725;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Akira Ikari, Chiaki Okude, Hayato Sawada, et al. (2008) Down-regulation of TRPM6-mediated magnesium influx by cyclosporin A Naunyn-Schmiedeberg's Archives of Pharmacology, 377(4-6):333-43.
This article references the use of the #21021 in the following applications :Western blotting

• Western blot analysis of extracts from 293 and Hela cells using c-Jun(Ab-91) Antibody #21021.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Ab-91) Antibody #21021(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using c-Jun(Ab-91) Antibody #21021.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting