• Product NameDynactin 1(N-terminus) Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.3D5-C6-D5
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsELISA WB ICC
• Species ReactivityHu P Ms B
• Immunogen TypeRecombinant protein
• Immunogen DescriptionPurified recombinant human DCTN1 protein fragments expressed in E.coli.
• Target NameDynactin 1
• Alternative Names    P135, DP-150, DAP-150
• Accession No. Swiss-Prot:Q14203
  Gene ID:1639
• UniprotQ14203
• Gene ID1639;
• Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

• Predicted MW: 150kd
  Western blotting: 1:1000
  Immunocytochemistry:1:300
  

Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

• Western blot detection of DCTN1 in A2780,TF-1,HepG2,MCF7,A431&K562 cell lysate(1:1000 diluted).
  Predicted band size:150KDa
  Observed band size: 150KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting