• Product NameMyc(Ab-58) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Myc protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.56~60 (L-P-T-P-P) derived from Human Myc.
• Target NameMyc
• Alternative Names    c-myc
• Accession No. Swiss-Prot: P01106
  NCBI Protein: NP_002458.2
• UniprotP01106
• Gene ID4609;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The expression correlates with the proliferation state of the cells. Transcription is repressed in quiescent or terminally differentiated cells. Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. Myc therefore is an important positive regulator of cell growth and proliferation. Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression. Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates

Jin Z, et al. (2004) J Biol Chem. 279(38): 40209-40219.
Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101(24): 9085-9090.
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.

• Western blot analysis of extracts from MCF cells using Myc(Ab-58) Antibody #21034.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Ab-58) Antibody #21034(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting