• Product NamePEG10 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1E12-F12-C12
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsELISA WB IP
• Species ReactivityHu Ms Rb P
• Immunogen TypeRecombinant protein
• Immunogen DescriptionPurified recombinant human PEG10 protein fragments expressed in E.coli.
• Target NamePEG10
• Alternative Names    EDR, HB-1, Mar2, MEF3L, Mart2, RGAG3
• Accession No. Swiss-Prot:P27361
  Gene ID:5595
• UniprotP27361
• Gene ID5595;
• Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

• Predicted MW: 55kd
  Western blotting: 1:1000
  

This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation.

• Western blot detection of PEG10 in Hela cell lysates using PEG10 antibody (1:1000 diluted).
  Predicted band size:55KDa.
  Observed band size:55KDa
• Immunoprecipitation analysis of Hela cell lysates using PEG10 antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting